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For immunofluorescence analysis of tissue sections, paraffin sections are deparaffinized and rehydrated, then treated with 3% hydrogen peroxide at room temperature for approximately 10 min to inactivate endogenous peroxidase activity.Then, place the sections in a container with antigen retrieval solution, heat in an autoclave until full pressure is reached for 3 min, followed by blocking with 1% goat serum albumin at room temperature for 30 min

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PubMed , Age , vol

Central activating transcription factor 4 (ATF4) regulates hepatic insulin resistance in mice via S6K1 signaling and the vagus nerve