hepatic glutathione role in liver diseases and hepatotoxicity Frontiers | Glutathione: Pharmacological aspects

Stuart-Low, W

In some tissues, GGT can be induced

10.1016/j.biomaterials.2015.01.021 193 NavoneS

The eYFP cDNA fragment was PCR amplified and cloned into NdeI and EcoRI digested pF1 plasmid to make pF1-eYFP plasmid (Supplemental Figure S6)

The samples were alkylated for 45 min at room temperature, mixed with 15 L non-reducing Laemmli buffer (4% SDS, 120 mM Tris/HCl p H 6.8, 20% glycerol, 0.02% bromophenol blue) and subjected to 12% SDS PAGE

The dissected brains were incubated in 100 nM TMRE solution (Thermo Fisher Scientific) in DSM for 10 min and relative fluorescence intensity was quantified by top excite-read method through Synergy H1 reader