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The precipitates were washed by 1 mL pre-chilled 90% acetone aqueous solution twice and then re-dissolved in 100 L 100 mM tetraethylammonium tetrahydroborate (TEAB)

10.1016/j.mayocp.2019.01.001 Mayo Clin

Hold the syringe needle-up and tap gently to move any remaining bubbles toward the needle

Glucagon-like peptide-1 receptor agonist prevented the progression of hepatocellular carcinoma in a mouse model of nonalcoholic Steatohepatitis

Importantly, commonly used in vitro BBB models, such as transwell systems and endothelial cell lines including RBE4, bEnd.3, and hCMEC/D3 monolayers, have important limitations, as they may overpredict permeability and do not fully recapitulate key in vivo BBB features, including physiological shear stress and multicellular neurovascular architecture (Chaulagain et al., 2023)