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Pierce BCA protein assay kit was applied to measure the total protein concentration of cell lysates

Interestingly, in traumatic brain injury, GPx1 deficiency was associated with decreased mitochondrial bioenergetic capacity compared to wildtype mice, whereas transgenic GPx1 mice had no decrease in mitochondrial function [114] GPx1 deficiency also enhanced apoptosis and inflammation in a cold-induced brain injury model [115]

Foresta C, Flohe L, Garolla A, Roveri A, Ursini F, Maiorino M

Isoform-specific induction of a human aldo-keto reductase by polycyclic aromatic hydrocarbons (PAHs), electrophiles, and oxidative stress: implications for the alternative pathway of PAH activation catalyzed by human dihydrodiol dehydrogenase

doi: 10.1007/s00122-006-0275-5 134 MesekaS.MenkirA.BosseyB.MengeshaW

In the future, more drugs may be developed, but MAPK pathway inhibitors will remain key