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doi: 10.1111/j.1600-065X.2012.01150.x 190 StaleyCWeingardenARKhorutsASadowskyMJ

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Chicago Dutta, Sanchari Sinha Dutta

Phosphopeptide enrichment Two hundred micrograms of peptides were enriched for phosphopeptides using Ti-IMAC magnetic beads (Resyn Biosciences)

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Negative controls were prepared by preincubating 10 M GSH with 10 M CNBF to block the thiol group prior to exposure to DTNB and H 2 S or by treating the obtained GSSH/GS(S) n H samples with excess TCEP (100 M) prior to alkylation and LC-MS analysis