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Secondary antibody (Anti-Rabbit IgG antibody, Goat monoclonal: Millipore AP132) was diluted 1:100 in dig wash buffer and cells were incubated at RT for 1 h

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Further, treatment with antioxidants or anti-inflammatory agents such as sulforaphane 110 and helminth therapy, 111 respectively, can help some patients with autism

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These can result in different effects from those of, 9 -THC alone since 9 -THC, is a CB1 and CB2 receptor partial agonist while 9 -THCV, behaves either as a CB1 antagonist or, at higher doses, as a CB1 receptor agonist (Pertwee, 2008)