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The following day, cells were treated respective of their experiment after which cells were immediately collected by scraping the bottom of the well and pelleting the samples by centrifugation at 2500G for 5 min at room temperature

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Unlike other glutathione peroxidase family members, GPX4 displays a unique catalytic preference for peroxides embedded in biomembrane phospholipids, enabling it to directly eliminate the most destructive lipid peroxidation products located on cellular and organellar membranes, safeguarding membrane stability and function at the source

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These mechanisms are explored in depth in the following sections