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As previously described (Sooklert et al ., 2019), cells were cultured in 75T culture plates and were maintained in standard culture conditions, Dulbeccos modified eagles medium (DMEM) containing 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and incubated in a humidified atmosphere of 5% CO 2 in air at 37C

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10.1021/acs.analchem.1c03032

Currently, measuring cardiac troponin levels is preferred over CK measurements for diagnosing heart issues