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However, as projects transition toward advanced translational applications, the inherent stochasticity of physical entrapment and the strict 3 kb thermodynamic limit for cargo size often necessitate a shift toward more programmable, sequence-guided encapsulation strategies to ensure the stoichiometric occupancy and structural stability required for clinical-grade manufacturing

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The water volume affects concentration and injection volume, not pharmacokinetics

By incorporating the antigen as an intrinsic structural element of the particle, this approach provides precise spatial control over antigen display, high stoichiometric uniformity, and superior particulate homogeneityadvantages that are difficult to achieve with post-assembly conjugation or non-covalent methods ( E