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The initiation phase involves the generation of ROS, including free radicals like hydroxyl radicals (OH), superoxide anions (O 2 ), and non-radical oxidants like hydrogen peroxide (H 2 O 2 ) [48, 49]

We then built the isobologram of the corresponding dose-response curves with a fixed dose ratio of R and compared the expected mixture of the two doses that produced an effect equal to half of the maximal effect produced by the individual drugs and to the experimental value (see later in methods)

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Glucagon-like peptide 1 conjugated to recombinant human serum albumin variants with modified neonatal Fc receptor binding properties