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This can be attributed to GBMs aggressive growth pattern, adaptability, diffuse infiltration, and genetic heterogeneity, all of which lead to high recurrence rates [3]

While iron metabolism and lipid peroxidation trigger ferroptosis under pathological conditions, the precise mechanisms remain incompletely understood and warrant further investigation

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In contrast, the study of GSH-Pxs in prokaryotes remains underexplored, and they have been reported only in Streptococcus pyogenes , where a gsh-px mutant was associated to an impaired virulence in mice infection models [13]

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After two rounds of selection, gene deletions were confirmed by PCR on genomic DNA using primers binding ~400 base pairs up- and down-stream of the gene of interest