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BC-derived primary cells (2 10 cells per 10 cm dish) were transduced with infectious lentiviral particles at a multiplicity of infection (MOI) of 10 in the presence of 8 g/ml polybrene (Santa Cruz, CA, USA, #sc-134220) for 48 hours

SURF1 organoids displayed deregulated expression of morphogens, downregulation of bioenergetic genes (MT-CO3, PDK4, PPARGC1A), and upregulation of glycolytic and proliferative genes

D., Primeaux, C