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RNA was then extracted using a SteadyPure Universal RNA Extraction Kit (Accurate, Hunan, China) in accordance with the manufacturers instructions

Sterile saline, which is sterile saltwater, is often used for wound cleaning, contact lens rinsing, and certain types of injections

30 l of supernatant was transferred to autosampler vials

The implementation of a small-volume, whole blood stimulation assay for the assessment of vaccine antigen-specific responses that can be set up at the trial site using minimal equipment, followed by sample preservation in RNA stabilisers and storage or transfer to the investigating laboratory for downstream processing, facilitates immunogenicity testing in clinical and field trials