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3 mL bacteriostatic water gives ~3,333 mcg/mL (gradual)

This simple sequence of amino acid residues not only has the capability of tissue regeneration and the enhancement of collagen and glycosaminoglycans synthesis but also is able to increase nerve outgrowth and angiogenesis

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The separated proteins were then transferred onto a PVDF membrane (Merck Millipore) and blocked using skim milk for 2 h at room temperature

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