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ALT is the most commonly used biochemical indicator of liver injury, but lacks specificity (Amacher 1998

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PFOA initially modulates the Toll-like receptors (TLR), hence regulating the MyD88 and NF-B pathways to govern cytokine transcription and stimulate the immune system in zebrafish (33)

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Its mechanism is competitive inhibition within the NNMT catalytic pocket, blocking the methyl transfer from SAM to nicotinamide