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After 3 h, the supernatant was incubated with 30 l of protein G agarose Fastflow for an extra 1 h, washed extensively with lysis buffer containing 250 mM NaCl (spun at 3000 rpm for 1 min)

This would presumably rely on its spirosuccinimide moiety, which bears resemblance to both the glutarimide motif present in thalidomide analogs and the asparagine cyclic imide modification proposed to be a natural degron recognized by CRBN 38,39,40