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357 The in vitro culture of telomere-binding protein protection of telomeres 1 (Pot1) in HSCs has been demonstrated to maintain self-renewal capacity and regulate the activity of HSCs in elderly individuals, which has significant implications for the ex vivo cultivation of human HSCs

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These are important issues, but they need not deflect us from the focus of this protocol, which is to describe the practicalities of measuring the K D for a simple ligand-receptor interaction