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Among various alkylating agents, N-ethylmaleimide is identified as the most reliable for thiol stabilization in liquid chromatographymass spectrometry (LC-MS) workflows, while specific reagents such as monobromobimane or -(4-hydroxyphenyl)ethyl iodoacetamide (HPE-IAM) are required for persulfide and polysulfide detection

Mitochondrial dysfunction induces an imbalance between oxidation and antioxidation, mitochondrial DNA (mtDNA) damage, mitochondrial dynamics dysregulation, and changes in mitophagy