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On the contrary, it's effect is felt most when it's lacking
After pharmacological intervention in 96-well cell culture plates for 24, 48 or 72 h, the culture medium was removed and replaced with 0.1 mg MTT reagent (Beyotime, ST1537) per well dissociated in 100 L basal DMEM (Procell, PM150210)

Second cancers account for up to 18% of all incident cancers in the United States, with 8% of them resulting from radiotherapy
