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The method is based on the reduction of Fe 3+ - TPTZ (2,4,6-tripyridyl-s-triazine) complex (FRAP reagent) to the ferrous (Fe 2+ ) form

However, this study demonstrated that antioxidant vitamins did not show a statistically significant effect by themselves

The relative importance of the glutathione system compared to the more important thioredoxin system may be further amplified under acute oxidative challenge as thioredoxins or thioredoxin reductases may readily become limiting and/or oxidised and thus inactive [62], which would strongly limit the capacity of the thioredoxin pathway to act as a conduit of anti-oxidative electron flux

Hypotheses 144 , 110242 (2020)

Specifically, the catalytic activities of pyruvate dehydrogenase and -ketoglutarate dehydrogenase are critically dependent on the production of lipoic acid, a straight-chain fatty-acid containing two sulfhydryl groups