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Description
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The assay is based on reversed-phase high performance liquid chromatography with UV detection and simultaneous reduction of disulfide bound with tris(2-carboxyethyl)phosphine and the selective pre-column derivatization of the thiol group with 1-benzyl-2-chloropyridinium bromide
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The results showed that the Cr(VI) was efficiently reduced by oxalic acid at high reaction temperature and high dosage of oxalic acid
