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5 g total protein in 10 L buffer was added to 490 L assay buffer, incubated at room temperature and the increase in absorption at 240 nm was monitored for 10 min every 5 min

85,86 Others found that magnesium stabilizes the structure of ALP and regulates its catalytic activity by zinc

J Biochem Mol Toxicol 32(1):e22006 Burmaolu S, Ylmaz AO, Polat MF, Kaya R, Glin , Algl (2019) Synthesis and biological evaluation of novel tris-chalcones as potent carbonic anhydrase, acetylcholinesterase, butyrylcholinesterase, and -glycosidase inhibitors

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