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After 24 h, the cell samples were processed by lysis in RIPA buffer containing protease inhibitor, scraping, and centrifugation (12,000 g for 5 min, 4 C)

Steroids (2012) 77(4):30611

Thus, microglia not only produce inflammatory mediators that enter the bloodstream through pro-inflammatory signaling by CORT but also activate their apoptotic pathways, leading to programmed cell death after sustained immune activity

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The brain is particularly vulnerable to oxidative stress due to its high oxygen consumption, lipid-rich environment, and limited antioxidant defenses

V., From Krebs to Clinic: Glutamine Metabolism to Cancer Therapy, Nature Reviews Cancer 16, no