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Heart protective effects and mechanism of quercetin preconditioning on anti-myocardial ischemia reperfusion (IR) injuries in rats

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doi:10.1111/j.17457599.2006.00160.x Yang WH, Drouin MA, Herbert M, Mao Y, Karsh J

2 H-labeled metabolites in the mitochondria were extracted by adding 500 L of cold extraction buffer (methanol: acetonitrile: water = 2:2:1, v/v/v), followed by sonication for 2 min and centrifugation at 14,000 g for 5 min at 4 C