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Briefly, fecal samples were suspended in sterile distilled water by vigorous shaking, and the supernatant was transferred to a 0.5 ml tube containing glass beads (0.1 mm diameter), before being spun at 5,000 rpm for 3 min in a mini-bead beater (BioSpec Products, Bartlesville, OK, USA)

Cephalalgia 38 , 17591764 (2018)

C.SenA.CoxW

Hong Kong Med J

Role of acetyl-L-carnitine in the treatment of cognitive deficit in chronic alcoholism 29

Metabolites Extraction The fresh frozen yolk samples including the yolk nutrients and water (100 mg) were individually grounded with liquid nitrogen, and the homogenate was resuspended with prechilled 80% methanol and 0.1% formic acid by well vortex