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It is produced from specific nutrients: amino acids that form its structure (cysteine, glutamine & glycine), vitamins, and minerals that participate in complementary reactions during its synthesis process (vitamins B2, magnesium and selenium)

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doi: 10.1210/edrv.19.3.0334, 12

If endotoxin is present, the activated enzyme cascade cleaves the substrate, producing a color change (typically yellow) whose intensity is proportional to the endotoxin concentration

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J Infect Public Health 13(1):5867 Egwim E, Elem R, Hamzah R (2011) Proximate composition, phytochemical screening and antioxidant activity of ten selected wild edible Nigerian mushrooms