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Owing to the metabolic reprogramming of tumor cells, many nutrients, including glutamine, are necessary for tumor cells to maintain their rapid growth and high metabolic demand

Feature GST Pull-Down Immunoprecipitation Binding Moiety GST Tag Antibody (Protein A/G/L) Beads Glutathione Agarose Beads Protein A/G/L Agarose Elution Harshness Mild Harsh Specificity High Varies Experimental Requirement Cloning with GST Tag Antibody availability How to do a GST pull-down

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Due to difficulties associated with the overexpression of Mycobacterium smegmatis EgtE protein, the proposed EgtE functionality remained to be verified biochemically