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26 Nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase, xanthine oxidase, nitric oxide synthase (NOS), lipoxygenase, and mitochondrial enzymes generate O 2

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To activate the AIM2 inflammasome, THP-1 macrophages or peritoneal macrophages were primed with LPS (100 ng/mL) for 3 hr followed by (Poly(dA:dT), 2 g/mL) for 6 hr using LyoVec TM (InvivoGen, tlrl-patc) according to the manufacturers protocol