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B 877 (2009), 3405 - 3417 Disclaimer Concerning questions to literature applications please contact the cited author/s
10.1016/j.tcb.2017.01.005 Trends Cell Biol
For patients confirmed to need b12 supplementation, either approach works

doi: 10.1016/j.lfs.2020.117905
Cells were washed once with PBS, then centrifuge to collect the cells and discard the supernatant