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Handling Protocol: Avoid repeated freezethaw cycles after preparation

5.1.2 Hydrophobic and hydrophilic bile acids in oxidative stress Hydrophobic bile acids (BAs) such as cholic acid (CA) and deoxycholic acid (DCA) are closely associated with lipid peroxidation and hepatocellular injury

For generation of VLP containing plasmid of interest (scramble, GFP or shRNA), the day before transfection, cells were seeded on 10 cm culture plates to reach 70% confluence for transfection

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Treatment with B 12 injections is efficacious in restoring normal metabolism, alleviating symptoms, and reversing haematological and some neurological complications of deficiency.23 Anaemia usually resolves within six to eight weeks, but coexisting iron deficiency may be present and need oral iron supplementation7 and, in severe cases, parenteral iron infusion