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For chymotrypsin digestion the alkylated proteins were diluted to 1 M urea with 100 mM Tris and 10 mM CaCl2 buffer pH 8.0 (50 g of protease in 57 l added to 456 l of buffer) and treated with 2.5 g of chymotrypsin made fresh in 1 mM HCl

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Genes Genomics 45 , 581592 (2023)

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