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Because this step is so decisive, it has its own guide

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Mobile phase: A mixed solution of sodium dihydrogen phosphate and sodium octanesulfonate (3.0 g of sodium dihydrogen phosphate and 1.0 g of sodium octanesulfonate, dissolved in water and diluted to 500 mL, adjusted to pH 3 with phosphoric acid) : acetonitrile = 96 : 4 (volume ratio)