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The experimental design involved oral administration of treatments (gavage) before the induction of sepsis

doi: 10.1016/j.euroneuro.2018.12.015 53

[DOI] [PubMed] [Google Scholar] 135.Muller F.L., Liu Y., Van Remmen H

This released Ca 2+ activates the ERK pathway and leads to release of DAG

In certain instances, a reduced frequency of administration of intratympanic injections reduces the risk of permanent damage (e.g., perforation) to the ear drum
