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Scientific Research Scientific research indicates that drinking contributes to vitamin B12 deficiency, that supplementation can help restore levels of this nutrient, and that alcoholic liver injury results in the purging of vitamin B12 from the liver: Moderate drinking among healthy, middle-aged women produced a statistically significant reduction in blood serum levels of vitamin B12 (source) Supplementation in healthy women over 60 with other B-vitamins over 6 months reversed these nutrient deficiencies and decreased levels of homocysteine, a compound associated with cardiovascular disease (source) Alcohol consumption in healthy male volunteers reduces B vitamin concentration and raises levels of inflammatory compounds (source) In severe alcoholics with liver disease, levels of vitamin B12 in the blood increase as the injured liver fails to hold on to stores of this vitamin (source) Falsely high serum levels of this nutrient can be caused by alcoholic liver disease (source) Unfortunately, most research money in the U.S

To understand the underlying molecular mechanisms, the expression levels of 3-AR mRNA in both white and brown adipose tissue were assessed using reverse transcription followed by PCR and Southern blotting

I had to begin the whole procedure over again with a red face, sweaty hands and a determination to get it right the second time