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Tissue proteins (15 g) were separated by electrophoresis on a NuPAGE 4-12% Bis-Tris gel (Invitrogen, Carlsbad, CA, USA) and transferred to a PVDF membrane

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Although the mechanism of cell death seems to be apoptosis as demonstrated by the appearance of positive apoptosis markers such as PARP cleavage, the effectiveness of Fer-1 in reducing cellular damage may indicate that the rotenone-induced apoptosis of SH-SY5Y cells has some of the characteristics of ferroptosis

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As a contemporary cancer treatment strategy, Photodynamic Therapy (PDT) has evolved from its inception to its current medical applications, expanding into various fields including oncology, dermatology, and dentistry