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Successive decarboxylations (by uroporphyrinogen decarboxylase, UROD) remove four acetate side chains to yield coproporphyrinogen III, which re-enters the mitochondria for oxidative decarboxylation to protoporphyrinogen IX by coproporphyrinogen oxidase (CPOX), followed by aromatization to protoporphyrin IX by protoporphyrinogen oxidase (PPOX)

After 48 h, cells were passaged 1:2 into fresh media containing 3 g ml 1 puromycin (Gibco, A1113803)

For the analysis of U87 cells, the HC PL APO 40x/1,30 OIL CS2 oil immersion objective was used

Foods 8(12):597

Mechanistic studies have uncovered numerous mechanisms by which GPx1 provides a survival advantage in various cancers

Getting this decision right protects both the integrity of the peptide and the safety of repeated use