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When assessing drug efficacy for the treatment of glioblastoma multiforme (GBM), currently, the most reliable models are generated through direct injection of patient-derived cells or more frequently the isolation of glioblastoma cells endowed with stem-like features and orthotopically injecting these cells into the cerebrum of immunodeficient mice

The lysates were incubated on ice for 30 min, followed by centrifugation at 12,000 g for 15 min at 4 C

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Thus, such matrices provide a highly reliable and efficient tool for research in numerous fields, including biomedicine, environmental monitoring, and material science

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