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The cell pellets were resuspended in 800 L of ice-cold 80% (v/v) methanol, mixed with 0.35 g of 0.2 mm zirconia-silica beads (BioSpec Products Inc., Bartlesville, OK, United States) in 1.5 mL microtubes, and cooled on ice

Further large scale trials are needed to corroborate these promising effects

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For example, the LC-MS/MS method mentioned in the study can simultaneously quantify up to 30 carnitines and their analogs, covering the range of C0 to C18, and significantly improves the accuracy and throughput of quantitative analysis