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non-conservative amino acid substitution refers to the amino acids substitution of an amino acid in one class with an amino acid from another class for example, substitution of an Ala, a class II residue, with a class III residue such as Asp, Asn, Glu, or Gin

PMID 14702039

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Studies have examined NNMT inhibition in preclinical systems involving energy balance, adipocyte biology, and NAD related chemistry

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Free Radic Res 31:525534 Upadhyaya CP, Venkatesh J, Gururani MA, Asnin L, Sharma K, Ajappala H, Park SW (2011) Transgenic potato overproducing L-ascorbic acid resisted an increase in methylglyoxal under salinity stress via maintaining higher reduced glutathione level and glyoxalase enzyme activity