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Once activated, these responses continue even after the peptide is cleared from the system

[DOI] [PMC free article] [PubMed] [Google Scholar] 21.Costanzo M., Romeo A., Cisterna B., Calderan L., Bernardi P., Covi V., Tabaracci G., Malatesta M

Save a small aliquot from each step of the purification for analysis by SDS-PAGE to monitor the location of the fusion protein throughout the purification (see Notes 13 and 14)
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