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For immunodetection, the blots were incubated overnight in PBS containing 0.1% Tween-20 and 5% skim milk with the primary antibody

Statistical analysis Statistical analysis was performed by parametric one-way ANOVA with post hoc Newman-Keuls test and non-parametric Kruskal-Wallis and subsequent Mann-Whitney U -test to compare groups
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Molecular docking analysis also suggest that SL has a strong affinity for the TRPV1 protein complex and can act as an inhibitor by binding to the protein

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