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In more advanced disease, these changes become more pronounced, with decreased free T3, elevated TSH, and more profound disruptions in hormone binding, metabolism, and clearance[85,86]

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Cagrilintide's half-life of approximately seven days allows for flexible dosing windows (administration can be delayed by 12 days without loss of efficacy), but missed doses beyond five days require restarting the titration schedule to avoid rebound nausea

Additional 1.8 ml chloroform was added, samples were vortexed (2 min, 2500 rpm) and incubated for 1 h at 4 C with rotation (32 rpm)

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