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RPMI 1640 medium without l-glutamine, l-cysteine, l-cystine and l-methionine was used as the base medium for different medium conditions (MP Biomedicals, 1646454)
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At 24 h post-infection, a lysis buffer was added to the cells, and the cell lysates were subjected to luciferase assay and measured with Cytation3 (Biotek, Winooski, VT, USA)
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