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Altogether, these data suggest a role for epigenetics in Epitalon bioregulatory activities

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Chromatin was isolated by adding 100 L of prechilled lysis buffer (50 mm TrisHCl, pH 8, 10 mm EDTA, 1% SDS, and cOmplete mini protease inhibitor) to the protoplasts, incubating on ice for 10 min, and resuspending by gentle pipetting

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