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In parallel, the concentration of total protein per sample was also determined by a Bicinchoninic acid (BCA) assay (Pierce) using the manufacturers protocol

Ly PT, Wu Y, Zou H, Wang R, Zhou W, Kinoshita A, et al

The code is included in the chapter related to diseases of the blood and blood forming organs and disorders of immune mechanism

Guynn RW, Pieklik JR

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It therefore appears that the TS pathway gradually evolved from early eukaryotes like Giardia and Entamoeba to intracellular Apicomplexans like Plasmodium to a fully functional pathway in Kinetoplastids like Trypanosoma