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Similarly, WT and Mgst2 -deficient 129/Sv female mice (10 per group) were given a single ip injection of 5-FU (300 mg kg 1 ) at time=0 and observed for distress as above

Data availability The RNAseq datasets generated during the current study are available in the GEO repository (GSE145395)

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IGFBP-3 (IGF Binding Protein-3) What it is: IGFBP-3 is the primary carrier protein for IGF-1 in the bloodstream

High levels of stress were also reported to be connected with disruptions in serotonergic-related pathways, the volume of some brain areas such as the hippocampus and prefrontal cortex, and epigenetic changes in genes such as BDNF [49,50,51]

Mice were housed in a temperature-controlled facility (24 C) under a 12-h light/dark cycle with free access to standard laboratory chow and sterilized water