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In murine models, NNMT knockdown did not induce the accumulation of NAM in livers and adipose tissues, nor did it cause significant alterations in the intracellular levels of NAD + in hepatocytes ( + is not necessary for this regulatory process

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doi: 10.1152/japplphysiol.01077.2006

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25 Structuralactivity relationship (SAR) studies on 1-MQ showed that small substituents like methyl or amino groups on 1-MQ were tolerated, while bulky groups on 1-MQ lost binding affinity due to steric effects