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Immunoblot analysis Cells were lysed on ice for 1 h in RIPA buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.6, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, protease inhibitor cocktail (Sigma), 1 mM phenylmethylsulfonyl fluoride (PMSF) (AppliChem))
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