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Subsequently, free ammonium sulfate was removed by dialysis and a 2 mg/mL doxorubicin solution was added (at 60 0 C), to allow for the doxorubicin to exchange with ammonium and precipitate within the liposome core ("active-loading")

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Most side effects are mild and resolve on their own (injection-site reactions, mild headache, water retention)

PubMed CrossRef Lee EB, An W, Levin ME, et al