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Direct adaptation was carried out for Dynamis media (A2261501, Thermo Fisher Scientific), CDFortiCHO media (A1148301, Thermo Fisher Scientific) and HyClone CDM4PERMAb media (SH30871.02, Cytiva), with the cells directly being cultured in 100% of the alternative media from EX-CELL Advanced CHO Fed-batch media and passaged at least 4 times over 2 weeks (Figure 1b)

However, several questions remain to be addressed

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The compound has never received market authorization in Canada or anywhere else, and Metabolic Pharmaceuticals terminated clinical development in 2007 after the Phase IIb obesity trial failed

IGF-1 DES is a recombinant and truncated form of IGF-1 produced in E.coli and lacking the first three amino acids of the N-terminus of the full peptide and thus also known as IGF-1 Des(13) and IGF-1 (470)

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